abstract：BACKGROUND:In recent years, a large number of studies have been published on the performance of glucose monitors. The quality of these reports is not known. METHODS:We searched the PubMed database for performance evaluations of handheld glucose monitors published from August 2002 to November 2006. Relevant articles we...

journal_title：Clinical chemistry

authors： Mahoney J,Ellison J

更新日期：2007-06-01 00:00:00

abstract：BACKGROUND:Institutions face increasing charges related to molecular genetic testing for neurological diseases. The literature contains little information on the utilization and performance of these tests. METHODS:A retrospective utilization review was performed to determine the diagnostic yield of neurogenetic tests ...

journal_title：Clinical chemistry

authors： Edlefsen KL,Tait JF,Wener MH,Astion M

更新日期：2007-06-01 00:00:00

abstract：BACKGROUND:The American Diabetes Association task force on standardization of insulin assays in 1996 showed wide variation in assay bias. Newer assays are specific for insulin, with several now available on automated immunoassay analyzers. METHODS:In 2004, we compared 11 commercially available insulin assays by analyz...

journal_title：Clinical chemistry

authors： Manley SE,Stratton IM,Clark PM,Luzio SD

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Direct equilibrium dialysis and direct ultrafiltration free thyroxine (T(4)) assays rely on semipermeable membranes to exclude T(4)-binding serum proteins from dialysates and ultrafiltrates. The presence of these proteins in dialysates or ultrafiltrates will yield spuriously high free T(4) values when free T...

journal_title：Clinical chemistry

authors： Fritz KS,Wilcox RB,Nelson JC

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Previous studies have shown a single linear relationship between mean plasma glucose (MPG) and hemoglobin A(1c) (HbA(1c)). We examined the relationship in different treatment groups of patients with type 1 diabetes participating in the Diabetes Control and Complications Trial (DCCT). METHODS:Seven-point glu...

journal_title：Clinical chemistry

authors： Kilpatrick ES,Rigby AS,Atkin SL

更新日期：2007-05-01 00:00:00

abstract：BACKGROUND:Simple, reproducible assays are needed for the quantification of sphingolipids, ceramide (Cer), and sphingoid bases. We developed an HPLC method for simultaneous quantification of total plasma concentrations of Cer, glucosylceramide (GlcCer), and ceramide trihexoside (CTH). METHODS:After addition of sphinga...

journal_title：Clinical chemistry

authors： Groener JE,Poorthuis BJ,Kuiper S,Helmond MT,Hollak CE,Aerts JM

更新日期：2007-04-01 00:00:00

abstract：BACKGROUND:Although minimum regulatory standards exist for determining QC testing frequency, decisions regarding when and how to run QC samples are not standardized. Most QC testing strategies test control samples at fixed time intervals, often placing the samples in the same position on an instrument during subsequent...

journal_title：Clinical chemistry

authors： Parvin CA,Robbins S 3rd

更新日期：2007-04-01 00:00:00

abstract：BACKGROUND:Hyperhomocysteinemia (HHcy) has been linked to impaired left ventricular function and clinical class in patients with chronic heart failure. We hypothesized that HHcy stimulates myocardial brain natriuretic peptide (BNP) expression and induces adverse left ventricular remodeling. METHODS:We randomized 50 ra...

journal_title：Clinical chemistry

authors： Herrmann M,Taban-Shoma O,Hübner U,Pexa A,Kilter H,Umanskaya N,Straub RH,Böhm M,Herrmann W

更新日期：2007-04-01 00:00:00

abstract：BACKGROUND:Measurement of postimmunization antibody response to pneumococcal capsular polysaccharide (caps-PS) is the standard method to identify deficiency of antipolysaccharide antibody production. However, no standardized criteria have been defined for classification of patients into responders or nonresponders to c...

journal_title：Clinical chemistry

authors： Jeurissen A,Moens L,Raes M,Wuyts G,Willebrords L,Sauer K,Proesmans M,Ceuppens JL,De Boeck K,Bossuyt X

更新日期：2007-03-01 00:00:00

abstract：BACKGROUND:Hypoxic ischemic encephalopathy (HIE) is a major cause of permanent neurological disabilities in full-term newborns. We measured activin A in urine collected immediately after birth in asphyxiated full-term newborns, and assessed the ability of the measurements to predict the occurrence of perinatal encephal...

journal_title：Clinical chemistry

authors： Florio P,Luisi S,Moataza B,Torricelli M,Iman I,Hala M,Hanna A,Petraglia F,Gazzolo D

更新日期：2007-03-01 00:00:00

abstract：BACKGROUND:Subfractions of HDL, particularly large HDL (HDL2), are inversely correlated with the severity of coronary artery disease (CAD). alpha-Tocopherol (AT) is the main lipid-soluble antioxidant in plasma. Results of a previous small study (n = 44) suggested that either a combination of an antioxidant cocktail [80...

journal_title：Clinical chemistry

authors： Singh U,Otvos J,Dasgupta A,de Lemos JA,Devaraj S,Jialal I

更新日期：2007-03-01 00:00:00

abstract：BACKGROUND:Astilbin is a flavonoid isolated from the rhizome of Smilax glabra. In a previous study, we revealed its unique immunosuppressive activity, a selective inhibition against activated T lymphocytes. This characteristic of astilbin is beneficial for the treatment of human immune diseases. METHODS:We incubated a...

journal_title：Clinical chemistry

authors： Guo J,Qian F,Li J,Xu Q,Chen T

更新日期：2007-03-01 00:00:00

abstract：BACKGROUND:A simple point-of-care method for measuring leukocyte counts in a doctor's office or emergency room could be of great importance. We developed a protocol for measuring cell count by disrupting the cell membrane and analyzing specific proteins within the cells and used it to analyze proteins from eosinophils ...

journal_title：Clinical chemistry

authors： Rundström G,Jonsson A,Mårtensson O,Mendel-Hartvig I,Venge P

更新日期：2007-02-01 00:00:00

abstract：BACKGROUND:Little research has been done to examine whether gamma-glutamyltransferase (GGT) is prospectively associated with the development of chronic kidney disease (CKD). We performed a prospective study to examine the association between GGT and the risk for the development of CKD. METHODS:The study cohort include...

journal_title：Clinical chemistry

authors： Ryu S,Chang Y,Kim DI,Kim WS,Suh BS

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:One of the aims of cardiovascular genetics is to test the efficacy of the use of genetic information to predict cardiovascular risk. We therefore investigated whether inclusion of a set of common variants in candidate genes along with conventional risk factor (CRF) assessment enhanced coronary heart disease ...

journal_title：Clinical chemistry

authors： Humphries SE,Cooper JA,Talmud PJ,Miller GJ

更新日期：2007-01-01 00:00:00

abstract：BACKGROUND:The 2447 A > T pathogenic variation at codon 816 of exon 17 (D816V) in the KIT gene, occurring in systemic mastocytosis (SM), leads to constitutive activation of tyrosine kinase activity and confers resistance to the tyrosine kinase inhibitor imatinib mesylate. Thus detection of this variation in SM patients...

journal_title：Clinical chemistry

authors： Tan A,Westerman D,McArthur GA,Lynch K,Waring P,Dobrovic A

更新日期：2006-12-01 00:00:00

abstract：BACKGROUND:The coexistence of hypertension and dyslipidemia synergistically increases the risk of cardiovascular events. We investigated the effect of the lipid-lowering agent micronized fenofibrate on inhibition of carotid atherosclerosis in patients with essential hypertension and mild hyperlipidemia. METHODS:We mea...

journal_title：Clinical chemistry

authors： Zhu S,Su G,Meng QH

更新日期：2006-11-01 00:00:00

abstract：BACKGROUND:The resurgence of the parasitic disease schistosomiasis calls for more efficient diagnostic tests. We developed a rapid, simple, portable, and sensitive immunoagglutination assay that uses SiO(2) particles and quartz crystal microbalance (QCM) for quantifying Schistosoma japonicum (Sj) antibodies (SjAb). ME...

journal_title：Clinical chemistry

authors： Wang H,Zhang Y,Yan B,Liu L,Wang S,Shen G,Yu R

更新日期：2006-11-01 00:00:00

abstract：BACKGROUND:Genotyping methods that reveal single-nucleotide differences are useful for a wide range of applications. We used digestion of 3-way DNA junctions in a novel technology, OneCutEventAmplificatioN (OCEAN) that allows sequence-specific signal generation and amplification. We combined OCEAN with peptide-nucleic-...

journal_title：Clinical chemistry

authors： Amicarelli G,Adlerstein D,Shehi E,Wang F,Makrigiorgos GM

更新日期：2006-10-01 00:00:00

abstract：BACKGROUND:Hyperlipidemia associated with the protease inhibitor (PI) component of highly active antiretrovial treatment can lead to accelerated atherosclerosis. The apolipoprotein A-V (APOA5) gene, which affects VLDL production and lipolysis, may play a role in PI-induced hyperlipidemia, particularly in individuals wi...

journal_title：Clinical chemistry

authors： Guardiola M,Ferré R,Salazar J,Alonso-Villaverde C,Coll B,Parra S,Masana L,Ribalta J

更新日期：2006-10-01 00:00:00

abstract：BACKGROUND:Atherosclerosis is a systemic disease with focal rupture of vulnerable plaque responsible for major clinical events. Several population-based studies indicate an association between lipoprotein-associated phospholipase A2 (Lp-PLA2) and cardiovascular events. Lp-PLA2 is emerging as a biomarker that may be a p...

journal_title：Clinical chemistry

authors： Zalewski A,Nelson JJ,Hegg L,Macphee C

更新日期：2006-09-01 00:00:00

abstract：BACKGROUND:Variability among assays used to measure intact parathyroid hormone (iPTH) is of particular concern because of the routine use of iPTH assay results to guide management of osteodystrophy and calcium metabolism in patients with end-stage renal disease (ESRD). The aim of this study was to determine the extent ...

journal_title：Clinical chemistry

authors： Cantor T,Yang Z,Caraiani N,Ilamathi E

更新日期：2006-09-01 00:00:00

abstract：BACKGROUND:In clinical and forensic toxicology, general unknown screening is used to detect and identify exogenous compounds. In this study, we aimed to develop a comprehensive general unknown screening method based on liquid chromatography coupled with a hybrid triple-quadrupole linear ion trap mass spectrometer. MET...

journal_title：Clinical chemistry

authors： Sauvage FL,Saint-Marcoux F,Duretz B,Deporte D,Lachatre G,Marquet P

更新日期：2006-09-01 00:00:00

abstract：BACKGROUND:In vitro and animal studies suggest that tumor necrosis factor alpha (TNF-alpha) modulates intestinal iron transport. We hypothesized that the effect of TNF-alpha might be particularly relevant if iron absorption is not effectively controlled by the HFE gene. METHODS:In patients with homozygous C282Y hemoch...

journal_title：Clinical chemistry

authors： Krayenbuehl PA,Maly FE,Hersberger M,Wiesli P,Himmelmann A,Eid K,Greminger P,Vetter W,Schulthess G

更新日期：2006-08-01 00:00:00

abstract：BACKGROUND:Intraventricular hemorrhage (IVH) is a major cause of neurologic disabilities in preterm newborns. We evaluated the use of plasma activin A concentrations to predict the development of perinatal IVH. METHODS:We measured nucleated erythrocyte (NRBC) counts, plasma activin A, hypoxanthine (Hyp), and xanthine ...

journal_title：Clinical chemistry

authors： Florio P,Perrone S,Luisi S,Vezzosi P,Longini M,Marzocchi B,Petraglia F,Buonocore G

更新日期：2006-08-01 00:00:00

abstract：BACKGROUND:Suspension array technology has surpassed ELISA for automated, simultaneous detection and quantification of soluble biomarkers such as virus-specific antibodies. We describe assays in which antigens are attached to a lipid bilayer surrounding color-coded particles. METHODS:We used layer-by-layer technology ...

journal_title：Clinical chemistry

authors： Toellner L,Fischlechner M,Ferko B,Grabherr RM,Donath E

更新日期：2006-08-01 00:00:00

abstract：BACKGROUND:Several proteolytically derived fragments from the proline-rich region (PRR) of human inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4) have been identified by surface-enhanced or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS or MALDI-TOF-MS) as potential diseas...

journal_title：Clinical chemistry

authors： Song J,Patel M,Rosenzweig CN,Chan-Li Y,Sokoll LJ,Fung ET,Choi-Miura NH,Goggins M,Chan DW,Zhang Z

更新日期：2006-06-01 00:00:00

abstract：BACKGROUND:Endothelin-1 (ET-1), a potent vasoconstrictor, is difficult to measure because of its instability and its binding to receptors and plasma proteins. We report a rapid, robust way to indirectly quantify ET-1 release by measuring the C-terminal ET-1 precursor fragment (CT-proET-1) without an extraction step. M...

journal_title：Clinical chemistry

authors： Papassotiriou J,Morgenthaler NG,Struck J,Alonso C,Bergmann A

更新日期：2006-06-01 00:00:00

abstract：BACKGROUND:Formation of deposits of the insoluble amyloid beta-peptide is believed to be causally related with neurodegeneration in Alzheimer disease (AD). The beta-peptide originates from a larger amyloid precursor protein (APP) by the action of proteolytic enzymes. The first proteolytic event leading to amyloid forma...

journal_title：Clinical chemistry

authors： Verheijen JH,Huisman LG,van Lent N,Neumann U,Paganetti P,Hack CE,Bouwman F,Lindeman J,Bollen EL,Hanemaaijer R

更新日期：2006-06-01 00:00:00

abstract：BACKGROUND:Diagnosis of mitochondrial disorders usually requires a muscle biopsy to examine mitochondrial function. We describe our diagnostic procedure and results for 29 patients with mitochondrial disorders. METHODS:Muscle biopsies were from 43 healthy individuals and 29 patients with defects in one of the oxidativ...

journal_title：Clinical chemistry

authors： Janssen AJ,Trijbels FJ,Sengers RC,Wintjes LT,Ruitenbeek W,Smeitink JA,Morava E,van Engelen BG,van den Heuvel LP,Rodenburg RJ

更新日期：2006-05-01 00:00:00

abstract：BACKGROUND:We analyzed the low-dose (1 microg) rapid adrenocorticotropic hormone test (LDST) in 17 patients with a normal hypothalamic-pituitary-adrenal axis to determine reference intervals for the LDST on the basis of poststimulation cortisol increments. METHODS:We analyzed test results for 17 patients (14 females a...

journal_title：Clinical chemistry

authors： Doi SA,Lasheen I,Al-Humood K,Al-Shoumer KA

更新日期：2006-04-01 00:00:00

abstract：BACKGROUND:We describe a simple tetra-primer amplification refractory mutation system PCR (T-ARMS-PCR) for detecting MUTYH mutations, which are associated with colorectal adenomas and colorectal cancer. METHODS:We designed specific T-ARMS-PCR assays for 6 mutations (Y165C, G382D, 1395_7delGGA, Y90X, 1103delC, and R231...

journal_title：Clinical chemistry

authors： Piccioli P,Serra M,Gismondi V,Pedemonte S,Loiacono F,Lastraioli S,Bertario L,De Angioletti M,Varesco L,Notaro R

更新日期：2006-04-01 00:00:00

abstract：BACKGROUND:Platelet-derived microparticles (PMPs) are generally considered a marker of platelet activation in cardiovascular disease. We studied the extent to which PMP subpopulations parallel platelet activation in vitro and in vivo. METHODS:Using flow cytometry, we analyzed PMP subpopulations from resting and activa...

journal_title：Clinical chemistry

authors： van der Zee PM,Biró E,Ko Y,de Winter RJ,Hack CE,Sturk A,Nieuwland R

更新日期：2006-04-01 00:00:00

abstract：BACKGROUND:Mucopolysaccharidosis type II (MPS II) is a lysosomal storage disorder related to a deficiency in the enzyme iduronate-2-sulfatase (IDS). Clinical trials of enzyme replacement therapy are in progress, but effective treatment will require screening assays to enable early detection and diagnosis of MPS II. Our...

journal_title：Clinical chemistry

authors： Dean CJ,Bockmann MR,Hopwood JJ,Brooks DA,Meikle PJ

更新日期：2006-04-01 00:00:00

abstract：BACKGROUND:Reliable detection of neuroblastoma cells in bone marrow (BM) is critical because BM involvement influences staging, risk assessment, and evaluation of therapeutic response in neuroblastoma patients. Standard cytomorphologic examination of BM aspirates is sensitive enough to detect single tumor cells. Conseq...

journal_title：Clinical chemistry

authors： Swerts K,De Moerloose B,Dhooge C,Vandesompele J,Hoyoux C,Beiske K,Benoit Y,Laureys G,Philippé J

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND:In this study we demonstrate the technical application of flow cytometry and cell sorting combined with gene-rearrangement clonality profiling to detect and confirm minimal disease in 2 leukemia and 2 lymphoma cases. METHODS:Specimens with low percentages (0.05%-5%) of abnormal lymphoid populations were ide...

journal_title：Clinical chemistry

authors： Zehentner BK,Fritschle W,Stelzer T,Ghirardelli KM,Hunter K,Wentzel C,Bennington R,Hansen CL,Myerson D,Kalnoski M,Wells DA,Loken MR

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND:An association has been described between mortality in children with meningococcal disease and functional polymorphisms in the interleukin-1 (IL1) cluster. We undertook a multicenter study to evaluate associations of these polymorphisms in a Central European population. PATIENTS AND METHODS:The study involv...

journal_title：Clinical chemistry

authors： Endler G,Marculescu R,Starkl P,Binder A,Geishofer G,Müller M,Zöhrer B,Resch B,Zenz W,Mannhalter C,Central European Meningococcal Genetic Study Group.

更新日期：2006-03-01 00:00:00

abstract：BACKGROUND:To address the continuing need for the standardization of clinical human cardiac troponin I (cTnI) assays, NIST, with the assistance of the AACC/IFCC Cardiac Troponin I Standardization Committee, has developed a new certified reference material, Standard Reference Material (SRM) 2921: Human Cardiac Troponin ...

journal_title：Clinical chemistry

authors： Bunk DM,Welch MJ

更新日期：2006-02-01 00:00:00

abstract：BACKGROUND:Cardiac troponins are specific biochemical markers of myocardial injury used in the diagnosis of acute myocardial disease and cardiac risk stratification. To avoid misclassification of patients, troponin assays must demonstrate precision at the low end of the measuring range. We report our evaluation of the ...

journal_title：Clinical chemistry

authors： Lam Q,Black M,Youdell O,Spilsbury H,Schneider HG

更新日期：2006-02-01 00:00:00

abstract：BACKGROUND:Clinical assessment of vitamin D status often relies on measuring total circulating 25-hydroxyvitamin D3 (25OHD3), but much of each vitamin D metabolite is bound to plasma vitamin D-binding protein (DBP), such that the percentage of free vitamin is very low. We hypothesized that measurement of free rather th...

journal_title：Clinical chemistry

authors： Al-oanzi ZH,Tuck SP,Raj N,Harrop JS,Summers GD,Cook DB,Francis RM,Datta HK

更新日期：2006-02-01 00:00:00